?immunoassay

immunoassay發(fā)音

英：[?mj?n??'?se?]　　美：[??mj?no?'?se?]

英：　　美：

immunoassay中文意思翻譯

n. 免疫測定；免疫分析

immunoassay常見(jiàn)例句

1 、A novel sensitive electrochemical immunoassay method was proposed based on gold nanoparticle mediated biocatalytic deposition of platinum followed by stripping voltammetric determination.───發(fā)展了一種基于納米金介導生物沉積鉑并以鉑催化氫還原伏安法進(jìn)行檢測的高靈敏電化學(xué)免疫分析新方法。

2 、Immunoassay is a useful technique, antibody is the key of immunoassay.───免疫分析是一項應用廣泛的技術(shù),抗體是免疫分析的關(guān)鍵問(wèn)題。

3 、An indirect competitive enzyme-linked immunoassay(ELISA) was developed using polyclonal antibody to measure the residues of sulfaquinoxaline(SQ) in food.───利用抗磺胺喹惡啉(SQ)多抗建立酶聯(lián)免疫分析方法,以測定SQ在動(dòng)物源食品中的殘留。

4 、The purpose of this study was to explore the clinical value of the platelet antibody screening and typing in platelets transfusion by using microcolumn gel immunoassay (MGIA).───為了探討微柱凝膠免疫分析技術(shù)在血小板輸注中血小板抗體篩選和配型的臨床應用價(jià)值,運用微柱凝膠免疫分析技術(shù)檢測血小板抗原抗體反應,包括抗體篩檢、交叉配血。

5 、Intended use This immunoassay kit allows for the specific measurement of Mouse Procalcitonin, PCT concentrations in cell culture supernates, serum and plasma.───產(chǎn)品概述： 預期應用 ELISA法定量測定小鼠血清、血漿或其它相關(guān)液體中降鈣素原(PCT)含量。

6 、Abstract: Chemiluminescence Immunoassay (CLIA) is an efficient detection tool combined from chemiluminescent reactions and immunoassay.───文摘:化學(xué)發(fā)光免疫技術(shù)是化學(xué)發(fā)光與免疫測定結合起來(lái)的一種高效檢測手段。

7 、A novel electrochemical immunoassay method based on Au nanoparticle labeled immunocom-plex enlargement used for detection of complement C_3 was proposed in this paper.───報道了一種用金標復合物放大的計時(shí)電位法測定補體C_3的電化學(xué)免疫檢測方法。

8 、The bioactivity assay of PEG-TA1, Tyr-PEG-TA1 and 125 I -Tyr-PEG-TA1 were performed by an enzyme immunoassay (EIA) method.───利用酶免疫分析(EIA)方法鑒定PEG-TA1增加酪氨酸殘基前后以及Tyr-PEG-TA1經(jīng)125I標記前后免疫學(xué)活性的變化。

9 、The incretion secretion metrical technique is the important embranchment in clinical diagnosis presently,in which the magnetic separate enzyme immunoassay is the main technique.───內分泌激素測量技術(shù)是醫療診斷技術(shù)發(fā)展的重要分支,磁分離酶聯(lián)免疫技術(shù)是其中的主要測量方法。

10 、Methods The serum level of testosterone and estradiol were measured with radio immunoassay in 47men with rosacea.25healthy subjects were selected as control.───方法 采用放射免疫法對47例男性酒渣鼻患者血清睪酮、雌二醇進(jìn)行了測定，并以25例正常男性作為對照。

11 、Moreover, We assay ADM on serum of maternal peripheral blood using enzyme immunoassay(EIA).───另外,用酶免疫測定法(EIA)測定以上各組婦女外周靜脈血ADM濃度。

12 、Chemiluminescence Immunoassay (CLIA) is an efficient detection tool combined from chemiluminescent reactions and immunoassay.───化學(xué)發(fā)光免疫技術(shù)是化學(xué)發(fā)光與免疫測定結合起來(lái)的一種高效檢測手段。

13 、A number of techniques and methods have been developed for the detection of DNA adducts, such as 32P-postlabelling, immunoassay, immuno-CE/LIF, LC/MS, micro-scale separation coupled MS.───當前DNA加合物分析、檢測的方法主要包括：32P后標記法、免疫分析法、免疫毛細管電泳激光誘導熒光法、液相色譜串聯(lián)質(zhì)譜法、微分離-質(zhì)譜聯(lián)用法等。

14 、AFP-L3 is currently available in SGH.Test principle This assay employs the quantitative sandwich enzyme immunoassay technique.───實(shí)驗原理本試劑盒應用雙抗體夾心酶標免疫分析法測定標本中AFP-L3水平。

15 、John's Wort in rats.Cyclosporin concentrations in blood samples were determined by a specific monoclonal fluorescence polarization immunoassay.───利用單株抗體螢光偏極免疫分析法定量血中環(huán)孢靈之濃度。

16 、Methods: Fluorescence polarization immunoassay(FPIA) was used to measure the blood concentrations of ciclosporin and sodium valproate.───方法:采用熒光偏振免疫分析法監測環(huán)孢素A和丙戊酸鈉的血藥濃度,并對監測過(guò)程進(jìn)行預防性和回顧性質(zhì)量控制。

17 、Submandibular tissues of rats were incubated in vitro and different concentration of FSH was added.Enzyme link immunoassay was used to measure EGF and NGF in supernatant.───體外孵育大鼠下頜下腺組織并給予不同濃度FSH,應用酶聯(lián)免疫分析方法檢測上清液中NGF和EGF的含量。

18 、Serum FINS, FPG, TG, TC, FFA concentrations were measured with chemi-luminescence immunoassay methods.───化學(xué)發(fā)光法測HNS、FPG、TG、TC、FFA濃度。

19 、Method: The serum concentration of oral phenytoin, carbamazepine and valproic acid were determined in 26,74 and 144 epileptic patients by fluorescence polarization immunoassay, respectively.───方法:采用熒光偏振免疫法對26例服用苯妥英鈉、74例服用卡馬西平、144例服用丙戊酸鈉的患者血藥濃度監測結果及臨床療效分析、評價(jià)。

20 、SONG Shi-ping, TANG Guo-zhong, YANG Jianzhong.Experimental research on immunoassay on solid-phase avidin for Free thyroxine[J].Journal of Clinical Laboratory Science, 2003, 21 (1): 31 - 33.───[3]宋世平,唐國忠,楊建忠.固相親合素在游離甲狀腺素免疫檢測中的應用[J].臨床檢驗雜志,2003,21(1):31-33.

21 、This paper introduces the principle and design of Chinese enzyme immunoassay instrument,and emphatically discusses the design of nonlinear data processing model.───文中闡述了國產(chǎn)磁分離酶免疫測定儀的儀器原理及設計,對數據處理模型的設計進(jìn)行了重點(diǎn)討論。

22 、METHODS:Bacteria in the dental plaque of the anterior deciduous teeth of children were isolated and identified by colony morphology, biochemical test and immunoassay of serum agglutination.───方法:采用細菌分離、培養、生化試驗和免疫學(xué)血清凝集試驗對幼兒乳前牙牙菌斑內細菌進(jìn)行分離鑒定,分析病原菌的定殖情況以及與嬰兒期喂養方式的關(guān)系。

23 、The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).───應用反向間接血凝試驗（RPHA）、酶免疫測定（EIA）和聚合酶鏈反應（PCR）測定了家禽血清、卵黃和牛乳清中HBV標志物及人HBV-DNA。

24 、Methods The serum E 2 levels were measured by the microparticle enzyme immunoassay (MEIA) in 26 infertile women before and after receiving induced ovulation therapy.───方法 應用微粒子酶免 (MEIA)法檢測 2 6例不孕婦女接受超促排卵治療前、后的血清E2 水平 ;

25 、In this article, we describe methodologies needed inmanufacturing the immunoassay kit from the preparationof each component to the stabilization of thesystem constructed.───在這篇文章中，我們描述了制造免疫檢測試劑盒所需的方法學(xué)，從各種成分的準備到構造成的系統的穩定。

26 、Methods Serum ACA in 38 patients with DM were examined by enzyme-linked immunoassay(ELISA).We followed the trail of 17 patients with pos itive ACA.───方法應用酶聯(lián)免疫吸附法(ELISA)檢測了38例老年DM患者血清ACA，并對ACA**的17例患者進(jìn)行了隨訪(fǎng)。

27 、Methods The quantities and activity of the TFPI were measured in 38 women with ovarian cancer by using an quantitative enzyme linked sandwich immunoassay(ELISA) and Substrate chromatography.───方法:應用雙抗體夾心酶聯(lián)免疫吸附法及發(fā)色底物法對38例卵巢癌患者血漿TFPI抗原含量及活性進(jìn)行測定。

28 、The concentrations of follicle-stimulating hormone(FSH) , luteinizing hormone(LH) , estradiol(E2) and progesterone(P4) were tested by chemical luminescent immunoassay(CLIA).───小白泡(SW)直徑為1-2mm。 血樣采用化學(xué)發(fā)光法測定血漿中促卵泡素(FSH)、促黃體素(LH)、雌二醇(E_2)和孕酮(P_4)含量。

29 、Methods The serum concentration of valproic acid of 48 Petit mal children were determined by fluorescence polarization immunoassay(FPLA) .───方法 對48例癲癇失神性發(fā)作兒童患者服用丙戊酸,用熒光偏振免疫法(FPIA)監測血藥濃度.

30 、Methods:Serum IGF-1 concentration in 53 patients with essential hypertension and 16 healthy persons was measured by enzyme immunoassay (EIA).───方法 :采取酶免疫分析法測定了 5 3例原發(fā)性高血壓患者 (原發(fā)性高血壓組 )和 16例正常人 (正常對照組 )的血清IGF 1水平。

31 、The development of enzyme immunoassays for the determination of pyrethroid residues were reviewed,including hapten design,antibody preparation,and immunoassay establishment and applications.───對擬除蟲(chóng)菊酯類(lèi)單一農藥殘留和多殘留酶免疫分析方法的研究進(jìn)展進(jìn)行了綜述。

32 、Intended use This immunoassay kit allows for the specific measurement of Rat Thromboxane B2（TXB2）concentrations in serum and plasma.───ELISA法定量測定兔血清、血漿或其它相關(guān)液體中血栓素B2 （TXB2）含量。

33 、Methods Plasma HCMV-IgM antibody was detected with enzyme immunoassay (EIA) .───方法 運用酶免疫測定法(EIA)檢測職業(yè)獻血員中抗-HCMVIgM。

34 、CLIA,according to the labels,can be divided as Luminescent Immunoassay (LIA),Lumminescent Enzyme Immunoassay (LEIA) and Luminescent Cofactor Immunoassay (LCIA).───化學(xué)發(fā)光免疫技術(shù)可根據抗原抗體標記物的不同而分為發(fā)光物免疫測定、發(fā)光酶免疫測定和發(fā)光輔助因子免疫測定三大類(lèi)型。

35 、Methods The FK506 concentration was detected by micro-particle enzyme immunoassay(MEIA),blood glucose and serum lipid was determined by automatic biochemical system CX5.───方法微粒子酶免疫法(MEIA)測定FK506血藥濃度;酶法測定血糖、血脂,動(dòng)態(tài)觀(guān)察血糖、血脂與不同血藥濃度的關(guān)系。

36 、All 26 uninfected patients had a nonreactive serum enzyme-linked immunoassay.───所有的26人的血清酶聯(lián)免疫吸附試驗都為陰性。

37 、Telomerase activity was determined by the telomeric repeat amplification protocol enzyme-linked immunoassay (TRAP -ELISA).───應用TRAP-ELISA法測定腎上腺腫瘤中的端粒酶活性。

38 、The immunoassay of a radiolabeled substance,such as a hormone or an enzyme.───放射免疫測定用經(jīng)放射性物質(zhì)標記的物質(zhì)(如荷爾蒙或酶)進(jìn)行免疫測定

39 、Plasma CNP level was also monitored by the enzyme immunoassay (EIA) at 1 hour, 4h ours, 1 day, 2 day, 3 day and 6 days post-injury.───并同時(shí)采用酶免疫分析法(EIA),觀(guān)測傷后多時(shí)點(diǎn)的體內血漿CNP水平。

40 、Intended use This immunoassay kit allows for the specific measurement of human surfactant protein A (SP-A) concentrations in BALF, serum, and plasma.───產(chǎn)品概述： 預期應用 ELISA法定量測定人血清、血漿、肺泡灌洗液，細胞培養物上清或其它相關(guān)液體中SP-A含量。

41 、The peripheral serum FSH & LH levels were estimated by invtrion bioassay and E2 & T by immunoassay.───對E_2峰的上升速度的影響則以體穴組最大，體穴加耳穴組其次。

42 、Methods thyroid stimulating hormone (TSH) value was detected with time resolved fluorescence immunoassay (TRFIA).───方法應用先進(jìn)的時(shí)間分辨熒光免疫法 TRFIA檢測新生兒促甲狀腺素的濃度篩查 CH。

43 、The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single.───在本研究中使用三明治型免疫分析法(三層式)使用抗體檢測待測抗原，并對抗原作定量分析。

44 、Methods We useed model of swimming exhausition of rats and adopting immunoassay eazyme magnetic analysis(IEMA) method to measure the content of insulin ,C P, to test content of blood glucose and FFA in colorimetric analysis method.───方法 采用大鼠游泳力竭模型，分別用磁性微粒分離的免疫酶聯(lián)測定法（IEMA）測定血清胰島素、C肽，用比色法測定FFA、血糖，并觀(guān)察力竭時(shí)間和心率的改變。

45 、A voltammetric enzyme - linked immunoassay based on a newsystem of o - aminophenol (OAP) - H2O2 - horseradish peroxidase (HRP) hasbeen used in the detection of HRP and labelled HRP.───提出了鄰氨基酚（OAP）－H2O2-辣根過(guò)氧化物酶（HRP）伏安酶聯(lián)免疫分析新體系并用于測定HRP及HRP標注物。

46 、Scanning Detection System of UPT-Based Immunoassay Test Strip[J].───引用該論文 盧健,周蕾,趙永凱,黃立華,劉蕾,楊瑞馥,黃惠杰.

47 、In this paper, application of immunoassay technologies in detection of food contaminants such as pesticide residues, veterinary drug residue will be briefly reviewed.───免疫分析在食品安全檢測中對農／獸藥殘留檢測有著(zhù)廣泛的應用前景，對幾種主要的免疫分析技術(shù)進(jìn)行了綜述。

48 、Methods Using an quantitative enzyme linked sandwich immunoassay(ELISA)and substrate chromatography to measure the quantities and activity of the TFPI of 19 woman with carcinoma of endometrium.───方法應用雙抗體夾心酶聯(lián)免疫吸附法及發(fā)色底物法對19例子宮內膜癌患者血漿TFPI抗原含量及活性進(jìn)行測定。

49 、Collecting venous blood of pre-anesthesia(T0),1 hour(T1),3 hours(T2) after intubation and 4 hours(T3) after operation respectively,and the content of ET was then measured via radio immunoassay.───分別于**誘導前、全麻**管后1h、3h、術(shù)后4h采靜脈血,用放射免疫分析法測定內皮素含量。

50 、Utilizing the technique of immunoassay to detect the expression of transgenic plant is simple, sensitive, fast and credible.───利用免疫檢測技術(shù)進(jìn)行植物轉基因的表達檢測是一種簡(jiǎn)單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質(zhì)發(fā)生特異性免疫反應的抗體或抗血清。

51 、METHODS The SDC were determined by the fluorescence polarization immunoassay (FPIA)with TDx.───方法 采用熒光偏振免疫法 ( FPIA)測定 SDC。

52 、Soy isoflavone variety of detection methods,such as UV spectrophotometry,chromatography,chromatography and immunoassay method.───大豆異黃酮的檢測方法包括紫外分光光度法、色譜法、層析法和免疫檢測法等。

53 、Hapten and antiserum for parathion immunoassay───對硫**半抗原改造及其免疫抗體

54 、Fluorescence polarization immunoassay (FPIA) is comprehensively reviewed with 45 references.───對熒光偏振免疫分析技術(shù)的研究進(jìn)展作了詳細的評述。

55 、It's a combined optical biosensor and lateral flow immunoassay that uses both reflective surface pla*on resonance and antibodyantigen binding.─── 這個(gè)結合了光生物傳感器和側流免疫層析法 用到了反射面 等離子共振和抗體抗原結合

56 、Methods 10 kinds of bone marker were determined by enzyme immunoassay and electrochemiluminescence immunoassay in serum and urine of 164 healthy controls and 120 patients.───方法應用酶聯(lián)免疫測定法或電化學(xué)發(fā)光免疫法分別測定164名健康成年人和120例患者血清和尿液中10項骨代謝指標。

57 、Legionella antigen in urine was detected using enzyme immunoassay (EIA) method.───應用酶免疫測定 (EIA)方法檢測尿中嗜肺軍團菌抗原。

58 、Fluorescence polarization immunoassay was used to determine the blood concentration of cyclosporin.───利用螢光偏極免疫分析法檢測血中環(huán)孢靈濃度。

59 、Underlying problems in the application of immunoassay are highlighted and future trends of immunoassay development postdated.───探討了免疫分析技術(shù)存在的問(wèn)題和免疫分析的未來(lái)發(fā)展趨勢。

60 、Immunoassay techniques, which also use an antibody-capture methodology, are approaching the requisite speed but lack sensitivity: a sample containing at least several thousand copies of the organism is needed to identify an agent.───免疫測定法也利用了抗體結合原理，速度接近要求，但靈敏度差：為了鑒定生物制劑，樣本至少需要包含上千份有機體。

61 、The level of cAMP was measured by immunoassay KIT.───并用酶聯(lián)免疫法測定細胞內環(huán)**腺苷(cAMP)的濃度。

62 、METHODS: Serum IGF-1 concentrations in 53 patients with essentia l hypertension and 16 healthy persons were measured by enzyme immunoassay (EIA).───方法:采取酶免疫分析方法測定5 3例為原發(fā)性高血壓患者和16例正常人的血清IGF - 1水平。

63 、Methods Thyrotropin(TSH) concentration in dried-blood spot specimens on filter paper was dectected by enzyme immunoassay.───方法標本采集用干濾紙血片法,采用酶免法檢測血中促甲狀腺素濃度。

64 、Intended use This immunoassay kit allows for the specific measurement of human cytokeratin 19 (CK-19) concentrations in cell culture supernates, serum, and plasma.───產(chǎn)品概述： 預期應用 ELISA法定量測定人血清、血漿、細胞培養物上清或其它相關(guān)液體中CK-19含量。

65 、Methods:The level of Epos were checked by means of radio immunoassay in 38 cases of acute leukemia, 19 cases of deadly solid tumor and 15 cases of healthy children.───方法 :用放射免疫分析法測定 38例急性白血病 ( AL)、1 9例惡性實(shí)體瘤患兒及 1 5例健康兒童血清 Epo水平。

66 、An indirect competitive enzyme immunoassay with monoclonal antibody was established for measuring clenbuterol in urine or food.───在間接競爭酶聯(lián)免疫吸附法的基礎上,研制了一種可以檢測尿樣或食品中鹽酸克侖特羅的試劑盒。

67 、New Frontiers in Agrochemical Immunoassay───農業(yè)化學(xué)制品免疫測定的新前沿

68 、A kind of chemiluminescnet immunoassay instrument is introduced in the paper.The serial communication protocol and the communication program are described in detail.───介紹了化學(xué)發(fā)光免疫定量測量?jì)x的組成,重點(diǎn)闡述虛擬面板的設計、上下位機通信協(xié)議的制定和通信程序的設計。

69 、The immunoassay of a radiolabeled substance, such as a hormone or an enzyme.───放射免疫測定用經(jīng)放射性物質(zhì)標記的物質(zhì)（如荷爾蒙或酶）進(jìn)行免疫測定

70 、The accuracy of time-resolved fluorescence immunoassay (TRFIA) instrument depends on the anti-noise capacity of the measurement circuit for weak fluorescence signals.───摘要時(shí)間分辨熒光免疫分析儀的測量精度取決于其對弱熒光信號檢測電路的抗噪聲能力。

71 、Enzyme-Linked Immunosorbent Assay (ELISA) is an immunoassay technology established on the combination of the highly specific antigen-antibody reaction and the efficient catalysis of enzyme.───摘要酶聯(lián)免疫吸附是將抗原抗體反應的高度特異性和酶的高效催化作用相結合發(fā)展建立的一種免疫分析技術(shù)。

72 、Methods FT3, FT4 were determined by immunoassay system in 71 patients with hyperthyroidism and 32 healthy controls.TG, TCH, HDL-C and total protein were determined at the same time.───方法用免疫化學(xué)發(fā)光技術(shù)檢測71例患者和32例健康對照組血清游離T3(FT3)、游離T4(FT4)，以生化儀檢測甘油三酯(TG)、膽固醇(TCH)、高密度脂蛋白膽固醇(HDL-C)和總蛋白的含量。

73 、Methods Rats used in this experiment were injected immunosuppressant into their abdomen.The immunoassay was made after acupuncture and irradiation at Baihui point by semiconductor laser.───方法實(shí)驗中大鼠腹腔注射免疫抑制劑后,分別用針刺和激光療法進(jìn)行治療,然后測定大鼠NK細胞的活性。

74 、Immunoassay is the traditional method for analysis of substances in trace amounts,combining the specificity of immunoreactions with sensitivity of the tracer.───免疫分析是利用免疫反應的特異性和示蹤物的靈敏性進(jìn)行物質(zhì)超微量分析的傳統方法。

75 、The incretion secretion metrical technique is the important embranchment in clinical diagnosis presently, in which the magnetic separate enzyme immunoassay is the main technique.───摘要內分泌激素測量技術(shù)是醫療診斷技術(shù)發(fā)展的重要分支，磁分離酶聯(lián)免疫技術(shù)是其中的主要測量方法。

76 、Because the signal-to-noise ratio is an essential indicator of system performance, there should be an optimal concentration of antibody for a given immunoassay system.───因為信號-噪聲比例是系統性能的本質(zhì)指示，所以對一個(gè)給定的免疫檢測系統來(lái)說(shuō)存在一個(gè)最佳的抗體濃度。

77 、Method:The levels of cytokine and immunoglobulin in sera of 23 patiants with RP were measured by ELISA and immunoassay and were compared with the normal data published by our department of Laboratory.───方法:對23例RP患兒行外周血細胞因子及免疫球蛋白水平的檢測,并與我院檢驗科測定的正常值進(jìn)行比較。

78 、The chemiluminescence analysis and the chemiluminescence immunoassay (CLIA) are a kind of analysis technology of ultromicro biomatters.───化學(xué)發(fā)光分析法和化學(xué)發(fā)光免疫分析法是近期發(fā)展起來(lái)一項超微量生物活性物質(zhì)分析技術(shù)。

79 、Methods:The CEA,CA125,CYFRA21-1 contents of 71 cases of lung cancer,20 cases of benign lung disease and 20 cases of healthy controllers were detected by electrochemiluminescence immunoassay(ECLIA).───方法:采用電化學(xué)發(fā)光法分別檢測71例肺癌患者和20例肺良性疾病患者及20例健康者的血清中CEA、CA125、CYFRA21-1的含量。

80 、Methods: We examined and observed 52 patients with typhoid and 20 normal controls by bacterial culture, Widal reaction, and enzyme-linked immunoassay for detection of typhoid bacilli H antigen.───方法該文采用細菌培養、肥達反應、傷寒桿菌H抗原酶聯(lián)免疫檢測檢查52例傷寒患者,20例正常人,進(jìn)行對照觀(guān)察。

81 、There are three kinds of biosensors relevant to bioluminescence &chemiluminescence: immobilized enzyme sensor, immunoassay sensor and report gene sensor with specialized regulator.───基于生物及化學(xué)發(fā)光的生物傳感器主要有三類(lèi) :酶固化傳感器、免疫分子傳感器和特異調控因子誘導的報告基因傳感器。

黃曲霉的檢驗檢疫方法是怎樣的？

答： 薄層層析法　　薄層層析（thin-layer chromatography,tlc)是在黃曲霉毒素研究方面應用最廣的分離技術(shù).自1990年，它被列為aoac (association of official agricultural chemists)標準方法，該方法同時(shí)具有定性和定量分析黃曲霉毒素的功能. 液相色譜法　　液相色譜（liquid chromatography,lc)與薄層層析在許多方面具有相似性，二者互相補充.通常用tlc進(jìn)行前期的條件設定，選擇適宜的分離條件后，再用lc進(jìn)行黃曲霉毒素的定量測定. 免疫化學(xué)分析方法　　利用具有高度專(zhuān)一性的單克隆抗體或多克隆抗體設計的黃曲霉毒素的免疫分析方法，也是最常用的黃曲霉毒素檢測方法.這類(lèi)方法通常包括放射免疫分析方法（radioimmunoassay,ria),酶聯(lián)免疫法（enzyme-linked of immunosorbent assay,elisa)和免疫層析法（immunoaflinity column assay,ica).它們均可以對黃曲霉毒素進(jìn)行定量測定.　(1) 免疫親和柱-熒光分光光度法和免疫親和術(shù)-hplc法　免疫親和柱法和酶聯(lián)免疫吸附法雖然都可達到速簡(jiǎn)便效果，但酶聯(lián)免疫吸附法僅能檢測單一毒素（如黃曲霉毒素b1)含量，而且易出現假**結果，難以控制.免疫親和柱法（包括熒光光度法和hplc法）卻能達到既定量準確又快速簡(jiǎn)便的要求.　免疫親和柱的使用可以避免傳統tlc和hplc的缺點(diǎn)，同時(shí)免疫親和柱與tlc和hplc法結合可以大大提高工作效率，提高靈敏度和準確度.　黃曲霉毒素免疫親和柱-熒光光度計法是以單克隆免疫親和柱為分離手段，用熒光計，紫外燈作為檢測工具的快速分析方法.它克服了tlc和hplc法在操作過(guò)程中使用劇毒的 真菌毒素作為標定標準物和在樣品預處理過(guò)程中使用多種有毒，異味的有機溶劑，毒害操作人員和污染環(huán)境的缺點(diǎn).同時(shí)黃曲霉毒素免疫親和柱-熒光光度計法分析速度快，一個(gè)樣品只需10-15min,比傳統方法快幾個(gè)小時(shí)甚至幾天時(shí)間；儀器設備輕便容易攜帶，自動(dòng)化程度高，操作簡(jiǎn)單，直接讀出測試結果，可以在小型實(shí)驗或現場(chǎng)使用.可以進(jìn)行黃曲霉毒素總量 (b1b2g1g2) 的測定，檢測限可達到1ug/kg,達到黃曲霉毒素標準限量值以下測定范圍為1-300ug/kg.　黃曲霉毒素免疫親和柱-高效液相色譜法比傳統的hplc法更加安全，可靠，靈敏度和準確度高.它采用單克隆抗體免疫技術(shù)，可以特效性地將黃曲霉毒素或其他真菌毒素分離出來(lái)，分離效率和回收率高.　分析原理試樣中的黃曲霉毒素用一定比例的甲醇/水提取液經(jīng)過(guò)過(guò)濾，稀釋后，用免疫親和柱凈化，以甲醇將親和柱上的黃曲霉毒素淋洗下來(lái)，在淋洗液中加入溴溶液衍生，以提高測定靈敏度，然后用 熒光分光光度計進(jìn)行定量.也可以將甲醇-黃曲霉毒素淋洗液的一部分注入hplc中，對黃曲霉毒素b1,b2,g1,b2分別進(jìn)行定量分析.免疫親和柱是用大劑量的黃曲霉毒素單克隆抗體固化在水不溶性的載體上，然后裝柱而成.該方法的測定范圍0-300ug/kg.　(2) 酶聯(lián)免疫吸附法：　1996年，nakane 建立了 辣根過(guò)氧化物酶標記抗體的測定技術(shù).由于該方法簡(jiǎn)便，敏感，特異，可作為多種抗原或抗體的測定，20世紀70年代后期，該方法引入真菌毒素的檢測中，下面介紹的是競爭性酶聯(lián)免疫吸附間接法檢測黃曲霉毒素b1.　原理：將已知抗原吸附在固態(tài)載體表面，洗除末吸附抗原，加入一定量抗體與待測樣品（含有抗原）提取液的混合液，競爭培養后，在固相載體表面形成抗原抗體復合物.洗除多余抗體成分，然后加入酶標記的抗球蛋白的第二抗體結合物，與吸附在固體表面的抗原抗體結合物相結合，再加入酶底物.在酶的催化作用下，底物發(fā)生降解反應，產(chǎn)生有色物質(zhì)，通過(guò)酶標檢測儀測出酶底物的降解量，從而推知被測樣品中的抗原量.　(3) 微柱篩選法 可以用來(lái)半定量測定各種食品中黃曲霉毒素b1,b2,g1,g2的總量.　原理 樣品提取液中的黃曲霉毒素被微柱管風(fēng)硅鎂型吸附層吸附后，在波長(cháng)365nm紫外光燈下顯示藍紫色熒光環(huán)，其熒光強度與黃曲霉毒素在一定的光密度范圍內 成正比例關(guān)系.若硅鎂型吸附劑層未出現藍紫色熒光，則樣品為陰性（方法靈敏度為5-10ug/kg).由于在微柱上不能分離黃曲霉毒素b1,b2,g1,g2,所以測得結果為總的黃曲霉毒素含量.　(4) 一步式黃曲霉毒素檢測 金標試紙法　一步式黃曲霉毒素檢測金標試紙法是利用單克隆抗體而設計的固相免疫分析法.由此產(chǎn)生的一步式黃曲霉毒素快速檢測試紙可在5—10分鐘完成對樣品中黃曲霉毒素的定性測定.借助黃曲霉毒素標準樣品，這種方法能估算黃曲霉毒素的含量，非常適用于現場(chǎng)測試和進(jìn)行大量樣品的初選.

hr醫學(xué)上是什么意思

HR醫學(xué)上是心率的縮寫(xiě)，也是一個(gè)非常重要的醫學(xué)指標。通過(guò)測量心率，醫生可以判斷一個(gè)人的心臟健康狀況以及身體的代謝狀態(tài)。正常人的心率一般在60-100次/分鐘之間，如果超過(guò)或低于這個(gè)范圍，可能代表心臟存在問(wèn)題或身體系統出現異常狀況。

HRV醫學(xué)上是心率變異性的縮寫(xiě)，用來(lái)描述心率的變化規律。通過(guò)分析連續的心率數據，醫生可以確定一個(gè)人是否處于緊張、放松或者休息狀態(tài)。熒光素酶聯(lián)免疫分析(FIA,Fluorescence Immunoassay)是HRV的常用檢測方法。HRV可以反映一個(gè)人的自主神經(jīng)功能，是一個(gè)衡量身體是否處于最佳狀態(tài)的重要指標。

HR也是一個(gè)企業(yè)中經(jīng)常被提到的標準縮寫(xiě)，代表Human Resources，中文翻譯為人力資源。HR部門(mén)通常是一支專(zhuān)門(mén)負責企業(yè)管理、招聘、培訓等方面的團隊，是企業(yè)成功的關(guān)鍵之一。在企業(yè)管理中，HR的作用不可忽視，能為企業(yè)提供合適的人才，優(yōu)化組織結構和管理流程，提高企業(yè)的效率和競爭力。