?luciferase

luciferase發(fā)音

英：[lu:'s?f?re?s]　　美：[lu:'s?f??re?s]

英：　　美：

luciferase中文意思翻譯

n. [生]螢光素酶

luciferase常見(jiàn)例句

1 、A chemical substance present in the cells of bioluminescent organisms, such as fireflies, that produces an almost heatless, bluish - green light when oxidized under the catalytic effects of luciferase.───熒光素存在于生物性發(fā)光有機體（比如螢火蟲(chóng)）中的一種化學(xué)物質(zhì)，它在熒光素酶催化作用下氧化時(shí)發(fā)生幾乎沒(méi)有熱量的藍綠光

2 、DTA vector containing the binding sequence of EWS-FLI1 depresses the activation of luciferase───含EWS-FLI1結合序列的DTA載體對報告基因表達抑制的研究

3 、The fact that PTC124 binds to luciferase, he says, "has nothing to do with whether it's a good drug.───他認為，事實(shí)上PTC124連接上熒光素酶后，“并任何跡象顯示出它是一種好藥?！?/p>

4 、The luciferase activity of lysate expressing FLAG-pcDNA3-N was lower than that expressing FLAG-pcDNA3.Addition of N protein in RRL system decreased luciferase activity.───構建了載體FLAG-pcDNA3-N,在293T人胚腎細胞內表達后,熒光素酶活性被抑制; 在體外翻譯體系中加入N蛋白,體外翻譯被抑制。

5 、Approximately, 1.5- and 2.5-fold elevated levels of luciferase activity relative to untreated cells were detected in E. coli/pLux2 (UAG) and pLux3 (UAA) when gentamicin was present at 0.5 Ag/ml.───與未處理細胞相比，0.5Ag/ml濃度的慶大霉素能夠使pLux2(UAG)大腸桿菌和pLux3(UAA)大腸桿菌的熒光素酶活性提高1.5-2.5倍。

6 、All three mutants showed higher luciferase activity than that of the wild type, and the V85I mutant showed the highest activity.───三個(gè)突變異種均顯示比野生型具有更高的熒光素酶活性，并且V85I突變異種活性最強。

7 、96-Well Plate Dual Luciferase Assay───96孔板熒光素酶檢測

8 、Keywords Ewing family of tumor;fusion gene;diphtheria toxin A fragment （DTA）;luciferase;───尤文家族腫瘤;融合基因;白喉毒素A鏈;蟲(chóng)熒光素酶;

9 、Dr. Geri Dai, Promega Corporation Luciferase Gene Reporter System and Other Applications───利用螢光素酶做報告基因和其它分子生物學(xué)檢測

10 、Here, it was found that actin and luciferase nascent chains were adjacent to more than one TRiC subunit at different stages of translation.───盡管多方面表示混沌理論具有明顯的可應用性于現代企業(yè)問(wèn)題與供應鏈管理領(lǐng)域，然而，在這方面的應用仍是有限。

11 、In the natural world, a chemical called luciferin is mixed with an enzyme called luciferase.─── 在自然界中 一種叫熒光素的化學(xué)物質(zhì) 和熒光素酶混合

12 、Keywords lentivirus vector;luciferase;bioluminescence imaging;───慢病毒載體;熒光素酶;生物素熒光成像;

13 、Eriogyna pyertorum lucifera───n. 樟蠶四川亞種

14 、Keywords : Hepatitis C virus;Antisense oligodeoxynucleotide;Transgene;Luciferase;───肝炎病毒.丙型;寡核苷酸.反義;轉基因;熒光素酶;

15 、Expression of luciferase gene of pcDNA3 OCP Luc is specific to rhBMP 2 and can be used for the quantitative bioassay of rhBMP 2.───真核表達載體 pc DNA3- OCP- L uc的報告基因表達具有特異性 ,可代替直接測定骨鈣素用于 rh BMP- 2活性的定量測定研究 .

16 、Keywords glioma;mesenchymal stem cells （MSCs）;lentivirus;bioluminescence imaging （BLI）;luciferase;tropism;───膠質(zhì)瘤;骨髓間充質(zhì)干細胞;慢病毒;生物發(fā)光成像;熒光素酶;遷移;

17 、Establishment of cell model expressing HCV-C and luciferase fusion protein by microinjection───顯微注射法建立含熒光素酶和HCV-C基因細胞模型

18 、Most compounds identified by the firefly luciferase screen do not interact in this way with luciferase, he says, but it is important to make sure.───他認為，絕大多數的化合物能被熒光素酶識別，但不會(huì )發(fā)生和熒光素酶發(fā)生這種相互作用，但這必須得到確認。

19 、Keywords bioluminescent imaging;luciferase;polyethylenimine;promoter;suicide gene;ovarian cancer;───生物熒光成像;熒光素酶;聚乙烯基亞胺;啟動(dòng)子;自殺基因;卵巢癌;

20 、luciferase reporter gene activity───報告基因活性

21 、The constructed wild type p21 gene promoter driven luciferase repo rter plasmid is used to transfect ECV304 cells to express luciferase protein sta bly and reliably.───構建的野生型p21基因啟動(dòng)子驅動(dòng)的熒光素酶報告基因載體,轉染ECV304細胞后能夠表達熒光素酶蛋白,具有穩定和可靠性能。

22 、Keywords proline-rich nuclear receptor coactivator（PNRC）;luciferase assays;promoter characterization;───富含脯氨酸核受體輔活化子（PNRC）;熒光素酶活性檢測;啟動(dòng)子鑒定;

23 、18. The transcriptional activation activity of GR was evaluated through method of relative activity of luciferase.───相對熒光素酶活性法檢測GR轉錄激活功能的變化。

24 、I think we've mentioned them already, luciferin and luciferase.─── 就是之前已經(jīng)提到過(guò)的 熒光素和熒光素酶

25 、Laboratories can employ luciferase to emit light in the presence of certain diseases.───醫院用熒光素酶的發(fā)光來(lái)發(fā)現特定的疾病。

26 、Which, when catalyzed by luciferase, causes the bones to glow.─── 會(huì )被熒光素酶催化 讓骨頭發(fā)光

27 、Sites that are surrounded by parentheses are not unique, as additional sites for each also exists in the luciferase gene.───括號中的限制性?xún)惹忻肝稽c(diǎn)不是唯一的，因為他門(mén)在熒光素酶基因上也存在。

28 、Constructing and Identifying the Firefly Luciferase Report Gene Vectors For 5'Flanking Regulated Elements of NGAL Gene───NGAL基因5'側翼區轉錄調控元件螢火蟲(chóng)熒光素酶報告基因表達載體的構建與鑒定

29 、Banaiee N.Bobadilla del VM.Bardarov S Luciferase reporter mycobacteriophages for detection,identification,and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico 2001───呂斌.徐順清.符志軍.陳志飛.李開(kāi)賞.周宜開(kāi)重組分枝桿菌噬菌體檢測分枝桿菌的發(fā)光研究[期刊論文]-微生物學(xué)免疫學(xué)進(jìn)展2001(1

30 、Keywords NGAL gene;regulation of gene expression;luciferase;report gene;construction vector;───關(guān)鍵詞NGAL基因;基因表達調控;熒光素酶;報告基因;載體構建;

31 、Keywords RNA interference;luciferase;osteosarcoma;───RNA干擾;熒光素酶;骨肉瘤;

32 、prokaryotic luciferase───原核生物蟲(chóng)螢光素酶

33 、As leucine zipper is a basic DNA binding motif of many known transcription factors, we examined the transcriptional function of the TCP10L using dual luciferase assay system.───本研究的結果進(jìn)一步加深了對人體MT基因結構與功能的認識，也為闡明HCC發(fā)生的分子機制提供了有重要價(jià)值的資料。

34 、When paromomycin was used at 2.5 Ag/ml, luciferase activity was elevated 8- and 10-fold in E. coli/pLux2 (UAG) and pLux3 (UAA) relative to untreated cells.───當巴龍霉素的濃度達到2.5Ag/ml時(shí)，相對于未處理的對照組細胞，大腸桿菌/pLux2(UAG)和大腸桿菌/pLux3(UAA)的熒光素酶活性增加了8-10倍。

35 、Thus we constructed reporter vectors in which the reporter gene is luciferase.───經(jīng)酶切鑒定以及測序分析確定載體構建正確。

36 、Methods The human brain tumor cell line U87MG cells were transduced by using the SMPU-R-MND-luc vector then a clone with high luciferase activity was cloned and used for in vivo experiments.───方法用SMPU-R-MND-luc載體轉染人腦腫瘤U87MG細胞系,形成具有高熒光素酶活性的細胞克隆。

37 、Keywords gene transfer;electric pulse;luciferase;───基因轉移;電脈沖;熒光素酶;

38 、Keywords Dunaliella salina;luciferase gene;expression plasmid vectors;───杜氏鹽藻;熒光素酶基因;質(zhì)粒表達載體;

39 、Keywords dengue virus;replicon;Renilla luciferase;───登革病毒;復制子;海腎螢光素酶;

40 、fast luciferase automated assay of specimens for hospitals───醫院標本快速熒光素酶自動(dòng)測定

41 、The researchers developed a new screening system, based on a naturally fluorescent protein called luciferase, to identify small molecules that inhibit proteins that turn genes on and off.───在天然熒光蛋白熒光素酶的基礎上，研究者發(fā)展了一種新的篩選系統，此系統可以用來(lái)鑒定抑制啟動(dòng)和關(guān)閉基因蛋白的小分子。

42 、Addition of N protein in RRL system decreased luciferase activity.───在體外翻譯體系中加入N蛋白,體外翻譯被抑制。

43 、Keywords hepatitis C virus;cellular model;luciferase;ribozyme;───丙型肝炎病毒;細胞模型;熒光素酶;核酶;

44 、Light is emitted when luciferase is exposed to the appropriate luciferin substrate.Photon emission can be detected by light sensitive apparatus such as a luminometer or modified optical microscopes.───在熒光素酶中加入正確的熒光素底物就可以放出熒光，而發(fā)出的光子可以被光敏感元件，如熒光探測器或改進(jìn)后的光學(xué)顯微鏡探測到。

45 、cypridina luciferase───海螢螢光酶

46 、Luciferase can be used in blood banks to determine if red blood cells are starting to break down.───例如，熒光素酶可以被用于檢測血庫中所存血液中的紅血球是否開(kāi)始破裂。

47 、Luciferase expression in E. coli/pLux1 was not affected by paromomycin at sub-growth inhibitory concentrations.───反之，在低于生長(cháng)抑制的濃度下，巴龍霉素不影響大腸桿菌pLux1基因熒光素酶的表達。

48 、Keywords bioluminescence;total bacterial count;ATP;luciferin;luciferase;───生物發(fā)光;細菌總數;三**酸腺苷;熒光素酶;熒光素;

49 、Comparison of the relative luciferase activity in secondary CEF by different heterogenous strong promoters, MDV gB promoter and the composed promoters───啟動(dòng)子及其復合啟動(dòng)子的活性比較

50 、Keywords translation initiation region;luciferase;secondary structure;expression;───翻譯起始區;熒光素酶;二級結構;表達;

51 、Keywords PDGF-D;Single nucleotide polymorphism;Stroke;Haplotype;Promoter;Luciferase Report Gene System;───單核苷酸多態(tài)性;腦卒中;單體型;血小板源性生長(cháng)因子D;啟動(dòng)子;熒光素酶報告系統;

52 、Cotransfection of GalT I promoter /luciferase reporter and Ets-1 expression plasmid increased the luciferase reporter activity in a dose dependent manner.───我們采用熒光素酶報告基因的方法證實(shí)Ets-1可激活 GalT I報告基因的表達，并具有量效關(guān)系。

53 、30. The transcriptional activation function of GR was evaluated through the detection of relative luciferase activity after dexamethasone treatment.───相對熒光素酶活性法檢測地塞米松作用后GR轉錄激活功能的變化。

54 、ATP occurs wherever bacteria are present.Reaction of ATP with Luciferin Firetly in the presence of Luciferase results in the emission of light as a glow or flash.───ATP化驗ATP和蟲(chóng)熒光素就是螢火蟲(chóng)當閃動(dòng)的時(shí)候有細菌存在就有ATP存在。

55 、Inhibition of Luciferase expression in mammalian cells by AAV vector plasmid mediated Luciferase shRNA───AAV載體質(zhì)粒介導的熒光素酶shRNA抑制其在哺乳動(dòng)物細胞中的表達

56 、Keywords human telomerase reverse transcriptase（hTERT）;promoter;luciferase gene expression vector;───端粒酶催化亞基;啟動(dòng)子;熒光素酶基因表達載體;

57 、A chemical substance present in the cells of bioluminescent organisms,such as fireflies,that produces an almost heatless,bluish-green light when oxidized under the catalytic effects of luciferase.───熒光素存在于生物性發(fā)光有機體（比如螢火蟲(chóng)）中的一種化學(xué)物質(zhì)，它在熒光素酶催化作用下氧化時(shí)發(fā)生幾乎沒(méi)有熱量的藍綠光。

58 、Keywords Environmental pollutants;Electrophile chemical pollutants;Firefly luciferase;Transgenic cell;───環(huán)境污染物;親電性化學(xué)污染物;蟲(chóng)熒光素酶;轉基因細胞;

59 、bacterial luciferase───細菌熒光素酶, 細菌蟲(chóng)熒光素酶

60 、Renilla-type luciferase───花蟲(chóng)型熒光素酶

61 、However, as in the case of gentamicin, no increase in luciferase activity was observed in E. coli/pLux4 (UGA).───但與慶大霉素的研究結果相同，大腸桿菌/pLux4(UGA)的熒光素酶活性沒(méi)有增加。

62 、PCR. The MDK promoter/enhancer (MDKp) sequence with runt domain binding sites was amplified from the human genome DNA and directionally cloned into pGL3-Basic Luciferase reporter vectors to construct the MDKp-pGL3-B expression plasmids.───從基因組DNA中PCR擴增一段含Runt結合順序的MDK基因啟動(dòng)子/增強子序列(MDKp)，定向克隆到pGL3-Basic質(zhì)粒，構建MDKp-pGL3-Basic Luciferase報告基因表達載體；

63 、Inhibition of luciferase expression in hepatocellular carcinoma cells by short hairpin RNA───短發(fā)夾RNA抑制外源熒光素酶在肝癌細胞中的表達

64 、Gentamicin and paromomycin increased luciferase activity up to 2.5- and 10-fold, respectively.───抑制的作用機制很可能和通讀的增強有關(guān)，但此假設還需要進(jìn)一步驗證。

65 、Keywords Drosophila S2 cells;luciferase;promoter;reporter gene;transfection;───果蠅S2細胞;熒光素酶;啟動(dòng)子;報告基因;轉染;

66 、Then it was inserted into pBI-L vector to construct HGF and Smad7 expression vector containing luciferase reporter gene and respond element of tetracycline (TRE) by recombinant DNA technology.───利用DNA基因重組技術(shù)，將目的片段DNA**入含有熒光素酶報告基因和四環(huán)素反應元件的真核表達載體pBI-L中，分別構建真核表達載體pBI-L-HGF和pBI-L-Smad7，并進(jìn)行酶切和測序鑒定。

67 、Keywords low density lipoprotein receptor;luciferase reporter gene;Traditional Chinese Medicine;drug screening;───低密度脂蛋白受體;熒光素酶報告基因;中藥;藥物篩選;

68 、Cypridina-type luciferase───海螢火蟲(chóng)型熒光素酶

69 、Keywords PLAGL2;SP-C promoter;luciferase activity;measurement;EMSA;───PLAGL2蛋白;SP-C啟動(dòng)子;熒光素酶報告基因檢測;凝膠電泳遷移實(shí)驗;

70 、The details of cloning, expression and function of biotinylated luciferase will be discussed in this paper.───本文將就生物素化熒光素酶的克隆、表達以及功能檢測進(jìn)行具體討論。

71 、Since the mutant lossing the first 6 amino acids from the N terminus of firefly luciferase keeps 77% of natural luciferase activity, the 7th N terminal amino acid is of great importance to the normal enzymatic function.───由于N末端缺失6個(gè)氨基酸的突變體保持了77%的酶活性，因而螢火蟲(chóng)熒光素酶N末端第7個(gè)氨基酸與酶的催化活性密切相關(guān)。

72 、An alternative method uses bioluminescent proteins, such as the firefly enzyme luciferase, to detect pyrophosphate released when a base attaches to the primer strand.───另一種方法則利用了生物發(fā)光蛋白質(zhì)，像是螢火蟲(chóng)的螢光素酶（luciferase），來(lái)偵測核苷酸連接在引子序列后所釋放的焦**酸。

73 、9. 2. EWS-FLI-1 can regulate the expression of luciferase because of its powerful transcription activity.───尤文肉瘤特異性的EWS-FLI-1融合蛋白有強烈的轉錄活性，可以促進(jìn)蟲(chóng)熒光素酶基因高表達。收藏指正

74 、Keywords luciferase;bioluminescence;RLU;disinfection effect;rapid evaluation;───關(guān)鍵詞熒光素酶;生物發(fā)光法;RLU;消毒效果;快速評價(jià);

75 、Construction of expression plasmid vectors containing the luciferase gene of an algae Dunaliella salina───杜氏鹽藻熒光素酶基因表達載體的構建

76 、Keywords recombinant adeno-associated virus 2;firefly luciferase gene;transduction;gene therapy;───2型重組腺相關(guān)病毒;螢火蟲(chóng)熒光素酶基因;轉導;基因治療;

77 、The in vivo binding and functional relation between BRCA2 and FHL2 was demonstrated by coimmunoprecipitation, mammalian twohybrid assay and luciferase detection.───免疫共沉淀和哺乳動(dòng)物細胞雙雜交試驗顯示BRCA2和FHL2在體內特異性結合，并證實(shí)FHL2在體內形成同源二聚體；

78 、Construction of a luciferase report vector with prolatin regulating sequence───催乳素調控序列熒光素酶報告基因的構建

79 、EWS-FLI-1 can regulate the expression of luciferase because of its powerful transcription activity.───尤文肉瘤特異性的EWS-FLI-1融合蛋白有強烈的轉錄活性，可以促進(jìn)蟲(chóng)熒光素酶基因高表達。

80 、Renilla luciferase───海腎螢光酶

81 、Effects of Translation Initiation Region Secondary Structure on Expression of Luciferase Gene in COS-7 Cells───翻譯起始區二級結構對熒光素酶基因在COS-7細胞中表達效率的影響

82 、By measuring the quantities of luciferase and green fluorescence protein,we can validate whether the models are right or effective.───據此，我們初步認為，水溶成分中存在著(zhù)該種中藥的活性成分。

83 、Plasmids of the pCMV5-AML1/AML1-ETO and the MDKp-pGL3-B or mu-MDKp-pGL3-B were then co-transduced into 293T cells and the luciferase relative value was analyzed.───將正常和突變型表達載體與AML1或AML1-ETO基因瞬時(shí)共轉染293T細胞，分析/LML1、AML1-ETO對報告基因的轉錄調節作用。

熒光素酶發(fā)光原理？

利用熒光素酶與底物結合發(fā)生化學(xué)發(fā)光反,應的特點(diǎn)，把感興趣的基因轉錄的調控元件克隆在螢火蟲(chóng)熒光素酶基因(fireflyluciferase)的. 上游，構建成熒光素酶報告質(zhì)粒。

然后轉染細胞，適當刺激或處理后裂解細胞,測定熒光素酶活性。通過(guò)熒光素酶活性的高低判斷性刺前后或不同刺激對感興趣的調控元件的影響。

同時(shí)，為了減少內在的變化因素對實(shí)驗準確性的影響，將帶有海腎熒光素酶基因(Rinilla luciferase)的質(zhì)粒作為對照質(zhì)粒與報告基因質(zhì)粒共轉染細胞，提供轉錄活力的內對照，使測試結果不受實(shí)驗條件變化的干擾。

熒光素酶發(fā)光原理？

螢火蟲(chóng)熒光素酶在氧氣、ATP和鎂離子同時(shí)存在的條件下，催化熒光素氧化，生成氧化熒光素，同時(shí)產(chǎn)生黃綠色光，波長(cháng)540-600nm。

熒光素酶（luciferase）是自然界中能夠產(chǎn)生生物熒光的酶的總稱(chēng)。熒光素酶可以催化熒光素氧化成氧化熒光素，在熒光素氧化的過(guò)程中，會(huì )發(fā)出生物熒光。然后可以通過(guò)熒光測定儀測定熒光素氧化過(guò)程中釋放的生物熒光。熒光素和熒光素酶這一生物發(fā)光體系，可以極其靈敏、高效地檢測基因的表達，是檢測轉錄因子與目的基因啟動(dòng)子區DNA相互作用的一種檢測方法。

目前，最有代表性的是從甲蟲(chóng)中分離得到的螢火蟲(chóng)熒光素酶（Firefly luciferase）和從海腎中分離得到的海腎熒光素酶（Renilla luciferase）。