?HeLa

HeLa發(fā)音

英：　　美：

HeLa中文意思翻譯

Henrietta Lacks strain of cancer cells 海拉癌細胞株

HeLa常見(jiàn)例句

1 、TCS1 and TCS2 have no obvious effect on HepA-H cells, but have a significant inhibitory effect on HeLa cells, indicating that TCS2 is superior to TCS1 in anti-tumor activity by the way of inducing apoptosis.───HepA-H細胞對天花粉蛋白不敏感,而HeLa細胞對TCS1和TCS2敏感,其中TCS2抑癌活性明顯強于TCS1,細胞生長(cháng)受抑制作用顯著(zhù),作用機制與誘導細胞凋亡相關(guān)。

2 、Cytotoxic effect and radiation enhancement of docetaxel in uterine cervical carcinoma cell line Hela───多西紫杉醇對宮頸癌細胞系Hela細胞毒性和放射增敏作用的研究

3 、Hela first, then Manya threw down muff and satchel and began excitedly choosing their own apples.───先是海拉，接著(zhù)是瑪尼婭，都脫掉手套，放下書(shū)包，高興地挑選起自己要的蘋(píng)果來(lái)。

4 、The TEM and SEM observation of apoptotic Hela cells infected by HSV-1───HSV-1致Hela細胞凋亡的掃描電鏡及透射電鏡觀(guān)察

5 、incubation were fibroblasts,HeLa, BEL-7402,Detroit-6,SGC-7901,MGC 80-3 and Os-732 respectively according to the amount of ~3H-HPD in cells.───但在以后的24h 內,維持在類(lèi)似的水平上。 7株細胞溫育30min 攝入~3H-HPD 量,按多少順序依次為成纖維細胞、HeLa、BEL-7402、Detroit-6、SGC-7901、MGC 80-3 及 Os-732;

6 、Effects of ESAC on in vitro growth of 3 cell lines (including human hepatoma BEL 7402,Human cervix uteri tumor-derived HeLa and human umbilical vein endothelial cell line HUVEC) were determined by MTT method.───利用MTT法檢測ESAC對 3種細胞系 (人肝癌系BEL74 0 2、人宮頸上皮癌細胞系HeLa和人臍靜脈血管內皮細胞系HUVEC)體外生長(cháng)的影響。

7 、pE128 showed Rev dependent expression in Hela cell.───pE12 8在Hela細胞中顯示rev依賴(lài)性表達 ,prev不存在時(shí) ,幾乎沒(méi)有EGFP表達 ;

8 、STUDY ON CENTROMERIC DOTS OF HELA CELLS───HeLa 細胞染色體著(zhù)絲粒點(diǎn)變異研究

9 、THE COMPARISON OF SENSITIVITY OF BGM CELLS, HELA CELLS, HEL CELLS AND A 549 CELLS TO HERPES SIMPLEX VIRUS 1───BGM細胞,Hela細胞,HEL細胞與A549細胞對HSV-1敏感性的比較

10 、Radiosensitization effect of N-4-hydroxyphenyl retinode on cervical carcinoma cell line HeLa───4-羥苯基維胺脂對宮頸癌HeLa細胞系的放射增敏作用

11 、Hela cells were also injected to normal BALB/c and Kunming mice.───同時(shí)用Hela細胞作為**腫瘤細胞對照，并接種于免疫功能正常的BALB/c小鼠和昆明種小鼠。

12 、Experimental study of the low dose cisplatin enhancing the apoptosis of HeLa cell induced by radiotherapy───小劑量順鉑增強放療誘導HeLa細胞凋亡的實(shí)驗研究

13 、The inhibitory concentrations of iso-CPT to ECV304 and colo-16 cell were lower than those to tumor cells Tca8113 and Hela.───喜樹(shù)異堿抑制角質(zhì)形成細胞colo-16和血管內皮細胞ECV-304增殖的濃度小于抑制舌鱗癌Tca8113細胞和宮頸癌Hela細胞的濃度。

14 、Gpd-Tp92 fusion gene constructed in pcDNA3.1 (+) vector could express a fusion protein in Hela cells.───pcDNA3.1(+)/Gpd-Tp92在HeLa細胞中能有效表達。

15 、HEAT-KILL KINETICS IN CELL CYCLE OF HeLa CELLS───Hela細胞的熱療動(dòng)力學(xué)研究

16 、Hela said the remainder of our mortal lives won't last long.─── 海拉說(shuō)過(guò) 我們的生命不會(huì )持續太久

17 、Role of mitochondria in Tubeimoside I-mediated poptosis in human cervical carcinoma HeLa cell line───土貝母苷甲在人宮頸癌HeLa細胞內對線(xiàn)粒體的影響

18 、Molecular Cloning and Sequencing of P16 ink4 cDNA from Hela Cell───P16~(ink4)cDNA的克隆及序列分析

19 、Influence of Cardinal on Calcium Beaconage of Hela Cells───半邊蓮對Hela細胞鈣信號系統的影響

20 、RT-PCR showed that E1A gene had a stable transfection in Hela cells and obviously down-regulated Her-2 expression.───RT-PCR結果顯示:E1A基因在Hela細胞中能穩定表達,E1A基因的轉染明顯降低了Her-2基因在Hela細胞中的表達水平;

21 、The results of RT-PCT show that mRNA of p53 increase, while mRNA of p21 decrease then increase , and the results of Flow Cytometer show that HeLa cells accumulated in S-phase in one cell cycle.───RT-PCR結果顯示P53mRNA表達升高，而P21mRNA 表達為先降低后升高，并且流式細胞實(shí)驗結果顯示HeLa細胞在一個(gè)細胞周期時(shí)間內細胞主要積累在S期。

22 、MTT assay was used to examine the growth rates rates inhibition of AIDI on human hepatoma(BEL7402),human cervical carcinoma(HeLa),human melanoma(A375-S2),human gastric carcinoma(SGC-7901) and mice fibroma sarcomatosum(L_(929)).───體外實(shí)驗:常規傳代培養人肝癌BEL7402、人宮頸癌HeLa細胞、人黑色素瘤A375 S2細胞、人胃癌SGC 7901和小鼠纖維肉瘤L929細胞,用MTT法檢測活細胞數和生長(cháng)抑制率。

23 、uterine cervix eaneer cell HeLa───宮頸癌細胞

24 、Cervical Neoplasms Hela cell line───宮頸癌Hela細胞

25 、Determined by colonal formation, the inhibition rate of EWE (4mg/ml) against 7721, Hela, MKN-45 cells were 59.8%, 66.4%, 70.5%.───EWE4mg/ml對三種細胞集落抑制率分別為59.8%、66.4%、70.5%。

26 、With human tumor stem cell assay, CATC inhibited the stem cell growth of Hela and QGY7703 cell lines.───CATC對小牛主動(dòng)脈血管內皮細胞有明顯抑制作用。

27 、Keywords soybean trypsin inhibitor;the human hela cells;cell multiplication;───大豆胰蛋白酶抑制劑;人宮頸癌細胞;細胞增殖;

28 、Keywords S.acus Linnaeus exact;Hela;anti-cancer effect;MTT;───尖海龍提取物;瘤細胞株;倒置顯微鏡;抗癌作用;

29 、Detection of the Virus RNA from HeLa Cells Infected with? Coxsackievirus B_3 by in Situ RT-PCR───原位RT-PCR法檢測柯薩奇B_3病毒感染HeLa細胞中的病毒RNA

30 、Hela knew as soon as we tried to escape, our life force would start fading.─── 海拉知道 一旦我們嘗試逃離 我們的生命力就會(huì )開(kāi)始衰退

31 、OBSERVATIONS ON THE GROWTH DYNAMICS OF 4 STRAINS OF TOXOPLASMA GONDII IN HELA CELLS───四株弓形蟲(chóng)在HeLa細胞內生長(cháng)動(dòng)態(tài)的觀(guān)察

32 、Construction of Expressing Vector of Fusion Gene of EGFP and ALR and Its Expression in Hela Cells───EGFP和ALR融合基因表達載體構建及其在HeLa細胞中的表達

33 、Mechanisms of apoptosis induced by PBA-D1 in Hela cells───土槿乙酸衍生物PBA-D1誘導Hela細胞凋亡及其機制

34 、Keywords Chlamys nobilis;Glycosaminoglycan;Hela cell;inhibited effect;───華貴櫛孔扇貝;糖胺聚糖;人宮頸癌細胞;抑制作用;

35 、Obtainment and sequencing of the cDNA derived from the non-structural P2C of CVB4 First,we inoculated CVB4 into Hela cells which had been cultivated and spreaded the bottom of culture bottles.───一、柯薩奇B4病毒非結構蛋白P2C CDNA的獲取 首先采用細胞培養技術(shù)，當人宮頸癌細胞（HeLa ）長(cháng)滿(mǎn)后,接種柯薩奇B4病毒，細胞病變達到80%時(shí)及時(shí)收獲病毒。

36 、With FuGENE6 transfection reagent, AIRE gene was stably expressed in HeLa cells that would be helpful for further study on AIRE gene.───在 Fu GENE6轉染試劑的介導下 ,AIRE基因在 He La細胞內獲得穩定表達

37 、Construction of expression vector of follicular inhibin and HBsAg fusion gene and expression in Hela cell───卵泡抑制素與**表面抗原融合基因表達質(zhì)粒的構建及表達

38 、Effects of Smac gene over-expression on radiotherapeutic sensitivity of cervical cancer cell line HeLa───Smac基因過(guò)表達對宮頸癌HeLa細胞放療敏感性的影響

39 、Study on the Biological Properties of Solubilized HeLa Cell Receptors for Group B Coxsackieviruses───可溶性HeLa細胞柯薩基B組病毒受體性質(zhì)的研究

40 、but the inhibition rate was cut down to 28% in Hela and 13% in HepG2 cells lines.───但在Hela細胞中,抑制作用明顯減低至28%; 在HepG2細胞中抑制作用更弱,僅為13%。

41 、The telomerase activities of HeLa,Tca-8113,YTLMC-90 and Eca-109 cells in vivro were investigated by TRAP-ELIAS technique.───為了進(jìn)一步證實(shí)異鼠李素的抑癌抗癌作用，對其抑制腫瘤生長(cháng)增殖和誘導腫瘤細胞凋亡的分子機制進(jìn)行了實(shí)驗研究。

42 、human cervical carcinoma Hela cell───人宮頸癌Hela細胞

43 、Low dose cisplatin can enhance the sensitivity of radiotherapy through change cell cycle of Hela cell lines.───小劑量順鉑可通過(guò)改變培養的人Hela細胞周期 ,誘導細胞G2 /M期延遲從而提高放療敏感性 ,可能與其內在的 p5 3基因表達改變有關(guān)。

44 、Application of Microarray in Study on Apoptotic Mechanism of HeLa Cells Induced by HPV18 E6 siRNA and HPV Detection───基因芯片技術(shù)在HPV18 E6 siRNA誘導HeLa細胞凋亡機制研究及HPV檢測分型中的應用

45 、Hormesis and adaptive response of survival in HeLa cells induced by low dose X-ray irradiation───低劑量X射線(xiàn)照射誘導HeLa細胞存活的

46 、KGF and KGFR are expressed in Hela cells.───Hela細胞中有KGF和KGFR的表達;

47 、Keywords cell fusion;red blood cells;Hela cells;───關(guān)鍵詞細胞融合;雞紅細胞;聚乙二醇;

48 、Tubeimoside I-induced ultrastructural changes of human cervical carcinoma HeLa cell line and the protective effect of cyclosporine A───土貝母苷甲誘導的人宮頸癌HeLa細胞凋亡的超微結構變化及環(huán)孢菌素A的保護作用

49 、Effect of anticancer polypeptide from Buthus Martensii Venom (APBMV) on Eca 109 cells and Hela cells───東亞鉗蝎毒抗癌多肽對Eca109 細胞和HeLa 細胞的毒性作用

50 、Effect of the truncated human apoptosis-inducing factor expression on apoptosis of HeLa cells───人截短型凋亡誘導因子的表達對HeLa細胞的促凋亡作用

51 、Keywords Uterine cervix carcinoma;Proliferating cell nuclear antigen;Small hairpin RNA;Hela cell;───關(guān)鍵詞子宮頸癌:增殖細胞核抗原;小發(fā)夾結構RNA;子宮頸癌細胞;

52 、The objective was designed to investigate the function of VEGF-C in cervical cancer cell line Hela, proliferation, adhesion.───對腫瘤淋巴轉移的調控，是否也與其對腫瘤細胞自身增殖、黏附、侵襲等，轉移相關(guān)生物學(xué)行為的影響有關(guān)。

53 、Radiosensitivity and growth characteristics of filial generation from irradiated HeLa cells───HeLa細胞照射后存活后代生長(cháng)特性及放射敏感性的變化

54 、COMPARISON OF INTAKES OF ACM-LDL COMPLEX AND FREE ACM BY HELA CELLS OF UTERINE CERVICAL CARCINOMA───宮頸癌HeLa細胞攝取ACM-LDL及游離ACM的比較研究

55 、Stability of p21 WAF/CIP1 Regulated by Skp2 in HeLa Cells───HeLa細胞中Skp2的表達調控p21WAFCIP1穩定性

56 、ELEMENTARY DETECTION OF CORRELATION?BETWEEN PKA, PKC AND CDC2 ACTIVITY?IN G2/M/G1 PROGRESSING OF HeLa CELLS───HeLa細胞G2/M/G1進(jìn)程中PKA、PKC與CDC2活性變化相關(guān)性之初探

57 、Ang-2 cDNA were subcloned into pcDNA3 vector and transfected into Hela cell lines by the lipofection method.───將Ang-2的cDNA克隆到真核表達載體pcDNA3上，應用脂質(zhì)體轉染技術(shù)將該真核表達載體穩定轉染人Hela細胞。

58 、We found that the anti-HCMV-pp65-3 antibody from previously immunized animals cross react to unknown proteins from Hela extract.───在動(dòng)物實(shí)驗中，施打pp65-3 蛋白質(zhì)，得到抗pp65-3 抗體，會(huì )與Hela 抗原中的未知蛋白質(zhì)產(chǎn)生交叉反應。

59 、The Studies on Dynamic Relationship between HeLa Nucleolar rDNA Transcription, Pre-rRNA Processing and the Ultrastructure───HeLa細胞核仁rRNA基因轉錄和pre-rRNA剪切與超微結構動(dòng)態(tài)關(guān)系研究

60 、Determined MTT staining, the IC50 values of EWE against 7721, Hela, MKN-45 cells were 1.43, 1.67, 0.97mg/ml.───EWE對三種細胞降解MTTIC_(50)值分別為 1.43、1.67、0.97mg/ml;

61 、REVERSIBILITY OF THE EARLY G_1 BLOCKING OF THE ASYNCHRONOUS AND M-SYNCHRONIZED HeLa CELLS TREATED BY N-BUTYRATE───丁酸鈉對M期同步及非同步的HeLa細胞早G_1期阻斷的可逆性

62 、Suppressive effect on HeLa cells proliferation by phenothiazine derivatives alone and combining with ionizing radiation───吩噻嗪衍生物協(xié)同放射對HeLa細胞增殖的抑制效應比較研究

63 、Curcumin can induce apoptosis of HeLa cells to kill the tumor cells by suppressing the expression of HVP E6 to restore the function of P53.───姜黃素通過(guò)抑制HPV E 6的表達,恢復P 53的功能,引起細胞凋亡,起到殺傷腫瘤的作用。

64 、The Killing Effect of S. Acus Linnaeus Exact on Hela───倒置顯微鏡觀(guān)察尖海龍提取物對Hela細胞的殺傷作用

65 、Optimal Catalytic Conditions of HeLa Cell Telomerase and Reconstitution of Human Telomerase Activity───HeLa細胞端粒酶最適反應條件及活性重建

66 、To observe direct inactivate effect of PAP to CBV3 by microdose cytopathogenic effect inhibition assay in Hela cell cultures.───在Hela細胞上采用微量細胞病變抑制法觀(guān)察PAP直接滅活CBV3的作用。

67 、Effect of Bak on regulation of cell cycle and apoptosis in Hela cells───Bak基因對Hela細胞的細胞周期和細胞凋亡的調控作用(英文)

68 、Either the cell growth inhibitory rate or the colony inhibitory rate of the HeLa cells was 100%(P0.05).───12 Gy的輻射劑量能夠完全抑制腫瘤細胞的增生(細胞生長(cháng)抑制率及克隆形成抑制率均為100%,P0.05)。

69 、Hela human cervical carcinoma cell───人宮頸癌Hela細胞株

70 、Utilized radioactive immunosorbent assay, cAMP accumulation and protein kinase A(PKA) activity in HeLa cells and the effects of AFP were detected on the concentration and activity in the cells pretreated with pertussis toxin (PTX).───再用放射免疫結合法分析在百日咳毒素 (pertussistoxin ,PTX)預處理前后AFP對細胞內環(huán)腺苷酸 (cAMP)濃度及細胞內蛋白激酶A(proteinkinaseA ,PKA)活性變化的影響 .

71 、Both genes were cloned into GFP expression vector pIRES2 EGFP and transfected into HeLa cells.───將上述基因克隆入綠色熒光蛋白(GFP)表達載體pIRES2-EGFP中,轉染人HeLa細胞,在熒光顯微鏡和電鏡下觀(guān)察轉染細胞的形態(tài)和結構。

72 、Establishment of a two-dimensional electrophoresis technique for proteome of hela cells───hela細胞蛋白質(zhì)組雙向電泳技術(shù)的建立

73 、Specific Suppression of HPV18 E6 Oncoprotein by RNA Interference in Hela Cells───RNA干擾技術(shù)特異抑制HPV18E6基因表達的研究

74 、The KGF and KGFR genes were expressed in the Hela cells. The KGF and KGFR proteins were expressed in the Hela cells.───從基因水平和蛋白水平證實(shí)KGF及KGFR在Hela細胞中有表達。

75 、Different phenotypes of P68 between tumouric cell li nes (HeLa, TC 3H 10) and non-tumouric cell lines (NIH3T3 and NC 3H 10 ) were observed, and we reasoned that overexpression of P68 might profoundly a ffect cellular growth properties.───以前的工作表明P68核蛋白在同一細胞系不同生長(cháng)階段的表型不同。 它在腫瘤細胞 (HeLa和TC3H10 )與非腫瘤細胞 (NIH3T3和NC3H10 )之間表型亦有明顯差異。

76 、Using PCR, we also obtained the cDNA sequence of PRPF8 C-terminal and using co-immunoprecipitation experiments, we confirmed the interaction between PAI-2 and PRPF8 in HeLa cells.───另外我們也獲得了PRPF8羧基端的部分序列，進(jìn)一步用免疫共沉淀的實(shí)驗證實(shí)，PAI-2與PRPF8在HeLa細胞內確實(shí)存在相互作用。

77 、The s-lap/GFP fusion protein can express in Hela cells and locate in entire cells, but express highly in the nucleus.───s- lap/ GFP融合蛋白可在人 Hela細胞中表達 ,并主要**于細胞核。

78 、Cloning of mouse Nanog gene and its effect on human Hela Cells───小鼠Nanog基因的克隆及對人宮頸癌上皮細胞的作用

79 、That electroporation can induce apoptosis is investigated in HeLa cells, the effects of electroporation to apoptosis of HeLa cells is studied with Giemsa,Acridine orange,Hoechst33342 and propidium iodide staining.───作者報道了電穿孔可以誘發(fā)HeLa細胞凋亡 ,采用Giemsa染料、吖啶橙染色及Hoechst33342 ,PI雙染的熒光染色 ,研究了電穿孔對HeLa細胞凋亡的影響 .

80 、Keywords P;Targeted radiotherapy;Radiobiological mechanism;HeLa cell lines;Apoptosis;Cell proliferation;Cell cycle;───關(guān)鍵詞32P;靶向治療;放射生物學(xué);細胞系;凋亡;細胞增殖;細胞周期;

81 、RT-PCR demonstrated that E1A gene had a stable transfection in Hela cells and obviously down-regulated HER-2/neu expression.───RT-PCR結果顯示:E1A基因在宮頸癌細胞中能穩定表達并且顯著(zhù)降低HER-2/neu在宮頸癌細胞中的表達水平。

82 、Human cervical carcinoma cell line HeLa───人宮頸癌細胞株HeLa

hela細胞高表達的基因有哪些

HeLa細胞通過(guò)關(guān)閉某些信號通路(包括PI3K和MKK4/MAPK信號通路),激活某些信號通路(包括cAMP、Ca~(2+)—CaM、PKC、Ras和P38/MAPK信號通路),激活或抑制不同的信號分子,使細胞產(chǎn)生具有針對性的細胞效應,包括誘導表達多種特異性蛋白(酶、細胞骨架蛋白、細胞因子等)、改變代謝途徑、減弱某些代謝、加強另一些代謝以及提高胞內運輸能力等,最終使自己可以適應痢疾桿菌的侵襲而生存下去。 為了鑒定在病原菌與寄主細胞相互作用中新的未知的EST序列,我們采用代表約4000個(gè)新基因的cDNA微陣列研究了痢疾桿菌入侵前和入侵后3h的人上皮細胞差異表達的新基因,結果共鑒定到≥3倍或≤0.33的差異表達的代表新基因的EST序列76條,然后這些EST序列采用Siclone軟件分析,進(jìn)行電子延伸,在BLAST比較分析之后,其中25個(gè)延伸序列鑒定為與重要的腸道功能相關(guān)的已知基因,包括痢疾桿菌-誘導的凋亡(TPPⅡ)、細胞骨架(如actin、talin、cofilin和gelsolin)、離子轉運(calpain-5、Nedd4和Calcium-transporting ATPaseⅠ)、離子通道功能(如ABCA8、TPD52等)、粘膜屏障防御(CLECSF2)、異型生物質(zhì)代謝以及上皮細胞的保護機制(vof-16)。更為重要性的是,通過(guò)RT-PCR克隆并測序,經(jīng)BLAST搜索,鑒定到3個(gè)顯著(zhù)差異的新的EST序列,并用熒光實(shí)時(shí)定量RT-PCR實(shí)驗證實(shí)這三個(gè)新序列的確在痢疾桿菌侵襲期間高表達。這三個(gè)基因是新的人基因或假基因,其相關(guān)信息已提交GeneBank,登錄號為nos.AY776160,AY776161和AY776162。我們認為它們在細胞對痢疾桿菌2457T侵襲反應中起重要的作用。這些結果促進(jìn)了對痢疾桿菌分子致病機理的認識,也為形成預防和治療痢疾的策略提供了理論基礎。

hela細胞轉染后幾小時(shí)死亡是什么原因

hela細胞轉染后幾小時(shí)死亡是什么原因

可能有多種原因： 目標蛋白對細胞有毒性，導致細胞死亡； 轉染試劑以及DNA用量信息需要優(yōu)化，否則對細胞具有傷害； 細胞貼壁轉染之后沒(méi)有正常換液。 建議： 考慮對目標蛋白進(jìn)行截短構建、嘗試其他細胞系統； 摸索轉染試劑以及DNA用量信息，如果轉染試劑毒性太大，可以考慮嘗試義翹轉染試劑sinofection； 對轉染后的細胞進(jìn)行換液處理，如果細胞狀態(tài)感覺(jué)不夠理想，可以考慮添加一些血清來(lái)幫助細胞恢復健康。 以上所有分析、建議的前提是，細胞培養、無(wú)菌操作等等都沒(méi)有問(wèn)題。祝順利，加油~