?fluorescein

fluorescein發(fā)音

英：[?flu:?'res??n]　　美：[?flu:?'res??n]

英：　　美：

fluorescein中文意思翻譯

熒光素

fluorescein詞形變化

動(dòng)詞第三人稱(chēng)單數: fluoresces | 動(dòng)詞現在分詞: fluorescing | 動(dòng)詞過(guò)去分詞: fluoresced | 名詞: fluorescer | 動(dòng)詞過(guò)去式: fluoresced |

fluorescein常見(jiàn)例句

1 、When CD 45 was conbined with other pairs of fluorescein isothiocyanate (FITC) and phycoerythrin (PE) conjugated reagents,it is possible to analysis on leukemic blasts and display dual parameter dats regarding expression.───CD45與異氰酸熒光素和藻紅蛋白標記的系相關(guān)抗體同時(shí)標記 ,能對白血病細胞群進(jìn)行多參數分析 ,更好地反應白血病細胞抗原表達特性。

2 、Methods The ocular manifestation of 25 patients (27 eyes) with PPSRH and their appearance of fundus fluorescein angiography ( FFA) were retrospectively analyzed.───方法對25例(27眼)PPSRH患者的眼部表現和眼底熒光血管造影(fundus fluorescein angiography,FFA)等臨床資料進(jìn)行回顧性分析。

3 、Visualization of CSF leaks is enhanced by coloring the CSF with fluorescein dye. CSF is then colored bright green and can be identified more easily.───利用熒光素染色腦脊液可以使泄露處更易顯示,此時(shí)腦脊液被染成明亮的綠色。

4 、Methods43eyes (42 cases) with contused wound were observed by fundus fluorescein angiography (FFA) and indocyanine green angiography (ICGA) at the same visited fundus.───方法用眼底熒光血管造影(FFA)和吲哚菁綠血管造影(ICGA)檢查本病的改變,共42例43只眼。

5 、Method Y-STR loci systems were apply to the cases in forensic science by using fluorescein labelling multiplex-polymerase PCR technique.───方法采用Y-STR熒光標記復合擴增技術(shù),結合案例應用。

6 、Methods Fudus photography, fundus fluorescein angiography (FFA) and indocyanime green angiography (ICGA) were performed in aseries of 34 cases with unilateral exudative AMD. The data of fluoroangiograms were analysed.───方法分析 34例經(jīng)ICGA確診的單眼滲出型AMD患者對側 34只眼的熒光素眼底血管造影(FFA)及ICGA圖像資料。

7 、Methods Morphological study,DNA gel electrophoresis,flow cytometry and fluorescein labelled annexin v were used to detect apoptosis,RT PCR to detect the apoptosis related gene.───方法 應用形態(tài)學(xué)、流式細胞術(shù)、DNA凝膠電泳、AnnexinV標記等方法檢測細胞凋亡的發(fā)生 ,應用RT PCR檢測凋亡相關(guān)基因Fas、bcl 2、c myc表達的變化。

8 、Method:16 patients (21 eyes) with acute retinal necrosis syndrome (ARN) were examined with fluorescein angiography (FFA),and the angiograms were analysed for the features of ARN.───方法 :用熒光素眼底血管造影方法檢查 16例 (2 1只眼 )急性視網(wǎng)膜壞死綜合征患者 ,分析其圖像特征。

9 、Methods The effects of ethylparaben on the principal agent fluorescein sodium and then on naturally contaminated germ were examined to check up the index of hygienics.───方法配制滴眼劑并加入相應防腐劑后,考察防腐劑對主藥的影響,然后自然染菌并做衛生學(xué)指標檢查。

10 、THE USE OF FLUORESCEIN DYE TO STUDY THE MOVEMENT OF WATER INTO WHEAT KERNELS───利用螢光染色跟蹤水份在潤麥時(shí)進(jìn)入小麥顆粒的路徑

11 、Methods Thirty-two eyes of 27 patients with AMD were selected for ICGA and fundus fluorescein angiographyh (FFA). The characteristics of the two examinations were compared for the difference.───方法 對 2 7例 (32只眼 )滲出型 AMD行 ICGA造影或 (和 )眼底熒光血管造影 (FFA)檢查 ,并對二者的造影表現特點(diǎn)和差異進(jìn)行比較。

12 、Methods:The results of fluorescein fundus angiography of 14 cases(16 eyes) with prepapillary vascular loops were analyzed retrospectively.───方法:回顧分析我院自開(kāi)展熒光素眼底血管造影以來(lái)的診斷為視盤(pán)血管袢的14例(16眼)臨床資料。

13 、A fluorometry method to determine cell viability by double staining with fluorescein diacetste and propidium iodide.Chinese Journal of Cell Biology[J].2000,22(1):50-53.───FDA-PI雙色熒光分光光度法檢測細胞活性變化[J].細胞生物學(xué)雜志,2000,22(1):50-53.

14 、Analysis and structural identification of the impurities in domestic fluorescein sodium injection───國產(chǎn)熒光素鈉注射液中雜質(zhì)的檢測及結構鑒定

15 、METHOD: The contents of fluorescein sodium in serum were analysed with fluorescent HPLC after separation with fluorescein sodium as trace agent.───方法 :利用熒光素鈉作為示蹤藥物 ,HPLC反相柱色譜分離原藥 ,熒光高效液相法定量分析血清熒光素鈉含量。

16 、Indocyanine green and fundus fluorescein angiography in anterior ischemic optic neuropathy───前部缺血性視神經(jīng)病變的吲哚青綠和熒光素眼底血管造影

17 、Fluorescein angiography showed leaking telangiectasia, macroaneurysm in the left eye but no abnormal leakage in the right eye.───左眼經(jīng)多次局部雷射光凝固治療后，最佳矯正視力可達零點(diǎn)陸。

18 、This paper reviews the characteristic of CCP and its function of delivery of proteins, antibodies, DNA, liposomes, fluorescein and imaging contrast agents in molecular imaging within cells.───本文就穿膜肽的特點(diǎn)，在功能上轉運蛋白、抗體、脂質(zhì)體、基因以及作為分子成像新途徑能結合熒光素，磁共振對比劑等進(jìn)行細胞內顯影作一個(gè)綜述介紹。

19 、FITC(fluorescein isothiocyanate)───異硫氰酸熒光素

20 、A new method of fluorescein isothiocyanate-labeled lipopolysaccharide───一種異硫氰酸熒光素標記脂多糖的新方法

21 、Fluorescein Derivatives Containing Reactive Group───含活性基熒光素衍生物

22 、Fluorescein angiography disclosed a sessile vascular lesion that filled with the retinal arterial circulation and leaked fluorescein into the subretinal space.───另有一環(huán)狀**之網(wǎng)膜下滲出物位于黃斑部之顳側。

23 、Conclusion: The side effects on fluorescein fundus angiography were maily concentrated on young and middle-aged male adults and ...───01)。結論:眼底熒光血管造影的不良反應發(fā)生率主要集中于中、青年男性,應針對性加強對高發(fā)人群眼底熒光血管造影前、后的護理。

24 、Methods Using A/B-scan ultrasonography (made in BVI, France), we examine 13 cases of ESMD, 7 cases of which were supported by fluorescein angiography(FA).───方法應用法國的BVI公司眼科專(zhuān)用A/B型超聲診斷儀，檢查13例滲出性老年黃斑變性患者。

25 、Fluorescein tear film break-up time───和螢光染色淚液膜分解時(shí)間

26 、Methods:Fundus fluorescein angiography(FFA) were performed in 8 patients(16eyes)of retinal degeneration.───方法:8例(16眼)視網(wǎng)膜變性患者按常規方法進(jìn)行眼底熒光血管造影。

27 、Methods: The retrobulbar circulation in 30 POAG patients (59 eyes) and thirty normal controls (59 eyes) were measured with color Doppler imaging (CDI). Fundus fluorescein angiography (FFA) was pictured in 10 patients (10 eyes) with POAG.───方法 :利用彩色多普勒成像 (color Doppler imaging,CDI)技術(shù)檢測 POAG患者與正常人各 30例 5 9眼的球后血流狀態(tài) ,其中 10例10眼行眼底熒光血管造影 (fundus fluorescein angiography,FFA)檢測。

28 、The data about the central vision, disease course, and results of fundus fluorescein angiography (FFA) of 99 patients (99 eyes) with CRAO were statistically analyzed.───對99例99只眼CRAO患者的中心視力、發(fā)病病程、熒光素眼底血管造影 (FFA)檢查視網(wǎng)膜循環(huán)時(shí)間等量化數值進(jìn)行統計學(xué)分析。

29 、Fundus fluorescein angiography(FFA)examinations were taken on 53 eyes in different time after operation.───其中53例在術(shù)后不同時(shí)間行眼底熒光血管造影檢查。

30 、Synthesis and Characterization of Rare Earth Complex with Fluorescein and 1,10-Phenanthroline[J].───引用該論文 雷思東,曹學(xué)偉,馮敏,李濤,王京,邵銘杰,周琦,王玉芳.

31 、Fluorescein isothiocyanate(FITC) is an important fluorescence probe.───異硫氰酸熒光素(FITC)是一種重要的熒光探針。

32 、METHODS Observe the alteration fundus lesions of pre-treatment and post-treatment by fundus color photography,fundus fluorescein angiography (FFA) and OCT in the 78 eyes of 46 patients with wet AMD.───方法確診為濕性AMD患者46例78只眼,觀(guān)察治療前后對患者的視力、眼底照相、熒光素眼底血管造影、OCT檢查情況。

33 、For ocular fundus images (OFIs), geometric distortion due to taking pictures at oblique visuai points and gray level distortion due to leakage of fluorescein and so on are almost unavoidable.───對于眼底圖像,由于不在正視點(diǎn)取像造成的幾何畸變和由于熒光素從血管壁滲漏等造成的灰度畸變,幾乎是不可避免的。

34 、Indocyanine green angiography and fundus fluorescein angiography in central serous chorioretinopathy───中心性漿液性脈絡(luò )膜視網(wǎng)膜病變的眼底血管造影

35 、The diffusion way and speed of fluorescein into the microcapsulations were observed by confocal laser scanning microscopy. The comparison of fluorescein intensity was made in APA microcapsulations with different diameters.───共聚焦顯微鏡下觀(guān)察葡聚糖熒光素在apa微囊中的**速度和**方式,并對比相同時(shí)間、不同直徑的apa微囊中葡聚糖熒光素的強度,綜合分析確定最佳的微囊直徑。

36 、Fundus fluorescein angiography findings in central serous chorioretinopathy───中心性漿液性脈絡(luò )膜視網(wǎng)膜病變的熒光眼底血管造影所見(jiàn)

37 、The PATH fluorescein in vitro method PATH,───體外熒光素方法、

38 、Influence of Stratum Corneum on the Percutaneous Absorption of Fluorescein Sodium Liposome in Rat Skin───大鼠皮膚角質(zhì)層對熒光素鈉脂質(zhì)體透皮吸收的影響

39 、In this paper, it was found that the weak ECL signal, generated from electrooxidation fluorescein on the surface of the carbon- printed electrode, was greatly enhanced by the chlortetracycline.───我們發(fā)現,堿性介質(zhì)中的熒光素在碳質(zhì)印刷電極表面的弱電化學(xué)發(fā)光信號可被金霉素所增敏。

40 、Methods:CDFI findings on 6 AMD cases were evaluated and compared before and after therapy and were supported by fluorescein angiography(FA).───方法 :對 6例 AMD患者治療前后的 CDFI結果做比較分析。 所有病例經(jīng)熒光血管造影 (FA)證實(shí)。

41 、In Situ Cell Death Detction Kit, Fluorescein───原位細胞凋忘檢測試劑盒熒光素法

42 、We present the clinical and fluorescein angiographic features of a case of unilateral Coats' disease with contralateral congenital retinal macrovessel.───右眼未做任何處置，病人無(wú)抱怨任何不適且視力可矯正至壹點(diǎn)零。

43 、Fluorescein Angiography;Computerized Visual Field Testing;───- 應診服務(wù): Digital Fundus Photography;

44 、MethodsRetrospectively analyzed the clinical data of fundus fluorescein angiography (FFA) and other related information of 1 521 patients who were diagnosed as DR.───方法 回顧分析 15 2 1例熒光素眼底血管造影 ( fundus fluorescein angiography,FFA)檢查確診的 DR患者的 FFA檢查結果、病程、視力等臨床資料。

45 、Keywords Chemiluminescence;Hypobromite;Hydrazine;Fluorescein;───化學(xué)發(fā)光;聯(lián)氨;次溴酸根;熒光素;

46 、Abstract: Fluorescein Isothiocyanate is a commonly used fluorescent dye to label chitosan, which is an important way to study the in vivo distribution, biodegradation and adsorption of chitosan.───摘要: 殼聚糖的熒光標記是研究殼聚糖在體內的分布、降解和吸收的重要方法之一。

47 、Acetylated dendrimers were conjugated to folic acid as a targeting agent and then coupled to either methotrexate or tritium and either fluorescein or 6-carboxytetramethylrhodamine.───將葉酸作為靶向物與**化樹(shù)枝狀結晶共軛，再將其與氨甲喋呤（或氚）和氫化熒光素（或6-carboxytetramethylrhodamine）配對。

48 、Keywords spectrophotometry;bovine serum albumin;tetrabromo（R）fluorescein;───分光光度法;牛血清白蛋白;四溴熒光素;

49 、Liu XL, Lin B, Qu J.Indocyanine green and fundus fluorescein angiography in central serous choriomopathy [J].Chin J Ocular Fundus Dis, 2000, 16(1): 14-6.───[2]劉曉玲,林冰,瞿佳.中心性視網(wǎng)膜脈絡(luò )膜病變的吲哚青綠和熒光血管造影[J].中華眼底病雜志,2000,16(1):14-6.

50 、It is significant to diagnose and treat central serous choroidoretinopathy with fluorescein fundus angiography.───作者認為熒光眼底血管造影對本病的診斷及治療均具有重要的指導意義。

51 、Indocyanine green fluorescein angiography plays an important role in fundus oculi diseases diagnosis.───吲哚菁綠造影技術(shù)對眼底疾病的診斷有很大的作用。

52 、Characteristics of clinic and fluorescein angiogram of juxtapapillary choroiditis───初盤(pán)旁脈絡(luò )膜炎眼底改變及熒光眼底血管造影的特征

53 、The image was obtained by taking multiple exposures through bandpass optical filter sets appropriate for fluorescein, Texas Red dye and DAPI using a 100X Plan Apochromat objective.───成纖維細胞分化為成軟骨細胞、成膠原細胞和成骨細胞，形成體內的纖維組織、肌腱、腱膜、各種支持組織和粘合組織。

54 、The number /mean area ratio of the fluorescein leakage had not a statistically significant difference among the early FFA phase on the 14,21 and 28 d(P>0.05),but a significant difference between the 14,21 and 28 d and the 7 d(P───光凝后FFA檢查經(jīng)組間兩兩比較,142、1和28 d的早期熒光滲漏斑數/滲漏面積差異無(wú)統計學(xué)意義(P>0.05),14、21和28 d與7 d相比差異有統計學(xué)意義(P

55 、Quenching and enhancement of fluorescence from rhodamine(RhB) and Fluorescein sodium(FS) molecules on the two colloids of silver nano-particles were recorded respectively and compared with each other.───當羅丹明B(RhB)分子分別吸附在這兩種納米銀上時(shí) ,對負電性納米銀 ,觀(guān)察到熒光猝滅、熒光峰紅移現象 ,且在分子的濃度適當時(shí) ,加入KBr可獲得較強的表面增強拉曼光譜 ;

56 、The pictures of 78 cases (86 eyes) of central serous choroidoretinopathy taken by fluorescein fundus angiography were anslyzed in this article.───本文通過(guò)熒光眼底血管造影檢查,對中心性漿液性脈絡(luò )膜視網(wǎng)膜病變78例(86眼)的造影圖象進(jìn)行了分析。

57 、Fundus fluorescein angiography for central serous chorioretinopathy───中心性漿液性脈絡(luò )膜視網(wǎng)膜病變眼底熒光血管造影

58 、Methods Fundus fluorescein an-giography (FFA) was made to determine the condition of fundus (optic nerve) in 64 cases of diabetic mellitus (127 eyes).───方法應用熒光素眼底血管造影(FFA),檢測64例127眼糖尿病患者眼底病變(視神經(jīng)病變)情況。

59 、More sensitive effect on dipolar SPR and larger band shift were observed.Great enhancement of fluorescence of the fluorescein molecules was obtained on the assembled structure of the silver nan...───在銀納米粒子組裝結構上,熒光素分子的熒光得到極大增強,其表面增強效應強烈依賴(lài)于組裝金屬粒子的表面等離子體共振。

60 、Forty-three eyes of 40 patients with active AMD lesions underwent OCT and simultaneous fluorescein and indocyanine green angiography (FA/ICGA) examinations were further investigated for the sources of subretinal neovascularization.───再選取同期做過(guò)OCT和同步FA/ICGA檢查的、處于病變活動(dòng)期的滲出型AMD患者40人43只眼，分析其視網(wǎng)膜下新牛血管(SRN)的起源。

61 、LABELING OF HUMAN SERUM ALBUMIN WITH FLUORESCEIN ISOTHIOCYANATE───異硫氰酸熒光素標記人血清白蛋白

62 、Methods 26 patients (48 eyes) with bullous retinal detachment were included in the study. An OCT scanning along with a fundus fluorescein angiography (FFA ) were performed in all patients.───方法對26例(48眼)臨床診斷為泡性視網(wǎng)膜脫離的患者,行FFA及 OCT檢查,并對所獲得圖像進(jìn)行分析。

63 、The authors reviewed the application of Fundus fluorescein angiography to the diagnosis,stage,evaluation of progress and treatment effect in diabetic retinopathy.───作者綜述了熒光素眼底血管造影在糖尿病視網(wǎng)膜病變診斷、分期、病情及療效判定中的應用。

64 、Methods 113 patients with CSCR were enrolled in the study.The fundus fluorescein angiography (FFA) showed leakage in all the selected patients.───方法將113例經(jīng)眼底熒光造影確診有明顯熒光滲漏點(diǎn)的中心性漿液性脈絡(luò )膜視網(wǎng)膜病變患者分為兩組。

65 、Analysis of ocular fundus and fluorescein angiography of Morning Glory syndrome───Morning Glory綜合征的眼底和熒光血管造影分析

66 、fluorescein labeled rabbit serum albumin───(異硫氰酸)熒光素標記兔血清白蛋白

67 、Fundus fluorescein angiography of bilateral central serous retinopathy───雙側性中心性漿液性脈絡(luò )膜視網(wǎng)膜病變熒光素眼底血管造影觀(guān)察

68 、Methods Eightyone patients (86 eyes) with CSC were performed by fundus fluorescein ang iography (FFA) and indocyanine green agiography (ICGA).───方法 對 81例 86只眼CSC患者應用熒光眼底血管造影 (FFA)和吲哚菁綠血管造影 (ICGA)結合臨床經(jīng)過(guò)進(jìn)行分析。

69 、FITC (fluorescein isothiocyanate) that can be linked to lipase as a fluorescence probe was used to study the relationships among the activity of lipase, conformation, and fluorescence spectrum.───摘要以FITC（異硫氰基熒光素）為熒光探針，研究了標記后的脂肪酶的活性、構象、熒光光譜的變化及三者之間的聯(lián)系。

70 、Theoretical investigations were performed to explore the electronic structure and optical properties of fluorescein using ZINDO, ab initio and DFT methods.───摘要用ZINDO、從頭算和密度泛函理論方法研究熒光素及其衍生物的電子結構和光譜性質(zhì)。

71 、FITC ( fluorescein isothiocyanat)───異硫氰基熒光素（FITC）

72 、fluorescein labeled antibody───吸蟲(chóng)

73 、Fundus autofluorescence (FAF) characteristics that are not identified by fundus photography or fluorescein angiography may serve as a prognostic determinant in advanced atrophic AMD.───未被眼底照相或熒光血管造影識別的眼底自發(fā)熒光的特征，可能是晚期萎縮性AMD的預后決定指標。

74 、METHODS Visual acuity and fundus were examined, and fundus fluorescein angiography(FFA) was performed in 121 eyes with pathological myopia.Data of these examinations were analyzed statistically.───方法對121只眼的高度近視患者作視力、眼底檢查及熒光素眼底血管造影檢查，并分析其臨床特點(diǎn)。

75 、In this patient, ready visibility of the retrobulbar blood vessels was made by performing ophthalmoscopy alone, without the aid of either fluorescein or indocyanine green angiography.───在此病患中，眼球后的血管可以直接單獨經(jīng)由間接眼底鏡的檢查而觀(guān)察到，不必透過(guò)眼底螢光或循血綠血管攝影的協(xié)助。

76 、Methods Thirty cases (32eyes) with CSC were examined by ophthalmoscopy,fundus fluorescein angiography and OCT.───方法 對30 例（32 只眼）中心性漿液性脈絡(luò )膜視網(wǎng)膜病變患者分別進(jìn)行檢眼鏡、熒光素眼底血管造影、OCT檢查。

77 、The clearity and lasting time of the development are two important indices to theoptical fundus blood vessel development of sodium fluorescein injecta. The fluorescencequenching directly affects the development quality.───使用熒光素鈉注射液進(jìn)行眼底血管造影,顯影清晰度和顯影持續時(shí)間是造影的重要質(zhì)量指標,熒光猝滅作用直按影響造影質(zhì)量。

78 、Methods Examined 116 cases(145 eyes) by direct ophthalmoscope,three mirror contact lens and fundus fluorescein angiography.───方法對116例(145眼)眼挫傷經(jīng)直接檢眼鏡和三面鏡檢查及眼底熒光血管造影檢查。

79 、Fundus fluorescein angiography (FFA) and Electroretinography (ERG) was taken before treatment, as well as 4h, 1d, 3d, 1week, 2weeks, 3 weeks, 4 weeks, 8 weeks, 12 weeks and 16 weeks after treatment.───于術(shù)前1d，術(shù)后4h、1d、3d、1w、2w、3w、4w、8w、12w和16w各時(shí)間點(diǎn)，分別進(jìn)行眼底熒光素血管造影(fundus fluorescein angiography，FFA)及視網(wǎng)膜電圖(electroretinogram，ERG)檢測。

80 、Covalently linking it to a fluorescent molecule, like fluorescein, without affecting it's.───作共價(jià)性接合,此接合反應不會(huì )影響其親和力。

鈣黃綠素屬于?；穯?/h2>

是的,屬于?；?。鈣黃綠素藍為無(wú)色結晶性粉末,易溶于堿性溶液,同時(shí)并產(chǎn)生持久的藍色熒光,微溶于水、乙醇及丙酮。鈣黃綠素藍可用作金屬熒光指示劑,在高ΟΙ時(shí)可用− Π / 0絡(luò )合滴定鈣,在低Ο Ι時(shí)可以絡(luò )合滴定銅、鎳和鉆,也可用于:一ΘΝ 0與重金屬結合的研究以及作為骨的熒光標記。氟化鏗一般用作干燥劑、助熔劑,也可用于光學(xué)玻璃制造、搪瓷工業(yè)、紅外線(xiàn)用的棱鏡和Τ光用的分光結晶等。,

中文名稱(chēng)：鈣黃綠素

中文別名：3,3'-雙(甲胺二乙酸)熒光素

英文名稱(chēng)：Calcein

英文別名：Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein;Fluorescein-bis(methyliminodiacetic acid);Fluorexon

CAS號：1461-15-0

分子式：C30H26N2O13

分子量：622.55

等級：AR

MDL號：MFCD00005049

Beilstein號：8181068

EC號：8181068

鈣黃綠素[1]

編輯本段性狀描述　　亮**粉末，溶于乙醇和堿，微溶于水；其鈉鹽為橙紅色結晶，溶于水，呈**而有綠色熒光，不溶于無(wú)水乙醇和乙醚。

編輯本段安全術(shù)語(yǔ)　　S26In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.

不慎與眼睛接觸后，請立即用大量清水沖洗并征求醫生意見(jiàn)。

S36/37/39Wear suitable protective clothing, gloves and eye/face protection.

穿戴適當的防護服、手套和護目鏡或面具。

編輯本段風(fēng)險術(shù)語(yǔ)　　R20/21/22Harmful by inhalation, in contact with skin and if swallowed.

吸入、皮膚接觸及吞食有害。

R36/37/38Irritating to eyes, respiratory system and skin.

刺激眼睛、呼吸系統和皮膚。

編輯本段用途說(shuō)明　　絡(luò )合指示劑；熒光指示劑；測定鈣、鍶、鋇、銅和錳

異硫氰酸熒光素可以把細胞染色嗎

異硫氰酸熒光素可以使細胞內的蛋白質(zhì)著(zhù)色進(jìn)而把細胞染色。

異硫氰酸熒光素是生化試劑,也是醫學(xué)診斷藥物,主要用于熒光抗體技術(shù)中的熒光染料,能和各種抗體蛋白結合,結合后的抗體不喪失與一定抗原結合的特異性,并在堿性溶液中具有強烈的綠色熒光。用于醫學(xué),農學(xué)和畜牧等方面,可對由地細菌病毒和寄生蟲(chóng)等所致疾病進(jìn)行快速診斷。

異硫氰酸熒光素(fluorescein isothiocyanate, FITC) FITC純品為**或橙**結晶粉末，易溶于水和酒精溶劑。有兩種異構體，其中異構體Ⅰ型在效率、穩定性與蛋白質(zhì)結合力等方面都更優(yōu)良。FITC分子量為389.4，最大吸收光波長(cháng)為490～495nm，最大發(fā)射光波長(cháng)為520～530nm，呈現明亮的黃綠色熒光。FITC在冷暗干燥處可保存多年，是目前應用最廣泛的熒光素。其主要優(yōu)點(diǎn)是人眼對黃綠色較為敏感，通常切片標本中的綠色熒光少于紅色。